A large sealed aerosol test chamber was used to replicate a potentially contaminated room environment and to contain any potential release of aerosols into the surrounding environment. The chamber was equipped with an NV900, filtered HEPA inlets, digital internal temperature and humidity monitor, external humidifiers (for humidity control), lighting system, multiple sampling ports, aerosol mixing fans, and an HEPA filtered exhaust system that were operated with a wireless remote control. For testing, the chamber was equipped with stainless steel probes for aerosol sampling, a port for bioaerosol dissemination into the chamber for the bacteriophages and vegetative cells, and a dry powder eductor for the fungal and bacterial spores.